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il 17f  (R&D Systems)


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    R&D Systems il 17f
    Figure <t>1.</t> <t>IL-17A</t> induced the up-regulation of endochondral ossification–related genes in the entheseal tissues. (A, B) Expressions of Col2A1 (A) and Acan (B) mRNA in the entheseal tissue culture. mRNA levels were determined using quantitative RT–PCR 72 h after IL-17A stimulation. (C) Production of the BMP2 protein in the entheseal tissue culture supernatant. The protein level was determined using <t>ELISA</t> 72 h after IL-17A stimulation. (D, E) Levels of mRNA (D) and protein (E) of FGF7 in the histoculture 72 h after IL-17A stimulation. The protein level of FGF7 in the supernatant of the entheseal tissue was measured by ELISA. (F, G) Effect of FGF7 on the expressions of Col2A1 (F) and Acan (G) mRNA in the tissue culture. The mRNA level was determined using quantitative RT–PCR 3 h after FGF7 stimulation (10 ng/ml). (H) Effect of FGF7 on the expressions of the BMP2 protein in the culture supernatant. The protein level was determined using ELISA 24 h after FGF7 stimulation (10 ng/ml). (I) Expression of IL-17RC mRNA in the histoculture. mRNA levels were determined using quantitative RT–PCR 24 h after IL-17A (10 ng/ml) or <t>IL-</t> <t>17F</t> (10 ng/ml) stimulation. The data presented are relative mRNA levels normalized to GAPDH. (A, B, C, D, E, I) Results are shown as the mean ± SD with individual data. *P < 0.05; **P < 0.01 by Dunnett’s parametric test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice. (D, E) *P < 0.05 by a t test or an Aspin–Welch t test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice.
    Il 17f, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+17f+duoset+elisa/pm39919800-237-9-10?v=R%26D+Systems
    Average 93 stars, based on 3 article reviews
    il 17f - by Bioz Stars, 2026-08
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    1) Product Images from "FGF7 as an essential mediator for the onset of ankylosing enthesitis related to psoriatic dermatitis."

    Article Title: FGF7 as an essential mediator for the onset of ankylosing enthesitis related to psoriatic dermatitis.

    Journal: Life science alliance

    doi: 10.26508/lsa.202403073

    Figure 1. IL-17A induced the up-regulation of endochondral ossification–related genes in the entheseal tissues. (A, B) Expressions of Col2A1 (A) and Acan (B) mRNA in the entheseal tissue culture. mRNA levels were determined using quantitative RT–PCR 72 h after IL-17A stimulation. (C) Production of the BMP2 protein in the entheseal tissue culture supernatant. The protein level was determined using ELISA 72 h after IL-17A stimulation. (D, E) Levels of mRNA (D) and protein (E) of FGF7 in the histoculture 72 h after IL-17A stimulation. The protein level of FGF7 in the supernatant of the entheseal tissue was measured by ELISA. (F, G) Effect of FGF7 on the expressions of Col2A1 (F) and Acan (G) mRNA in the tissue culture. The mRNA level was determined using quantitative RT–PCR 3 h after FGF7 stimulation (10 ng/ml). (H) Effect of FGF7 on the expressions of the BMP2 protein in the culture supernatant. The protein level was determined using ELISA 24 h after FGF7 stimulation (10 ng/ml). (I) Expression of IL-17RC mRNA in the histoculture. mRNA levels were determined using quantitative RT–PCR 24 h after IL-17A (10 ng/ml) or IL- 17F (10 ng/ml) stimulation. The data presented are relative mRNA levels normalized to GAPDH. (A, B, C, D, E, I) Results are shown as the mean ± SD with individual data. *P < 0.05; **P < 0.01 by Dunnett’s parametric test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice. (D, E) *P < 0.05 by a t test or an Aspin–Welch t test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice.
    Figure Legend Snippet: Figure 1. IL-17A induced the up-regulation of endochondral ossification–related genes in the entheseal tissues. (A, B) Expressions of Col2A1 (A) and Acan (B) mRNA in the entheseal tissue culture. mRNA levels were determined using quantitative RT–PCR 72 h after IL-17A stimulation. (C) Production of the BMP2 protein in the entheseal tissue culture supernatant. The protein level was determined using ELISA 72 h after IL-17A stimulation. (D, E) Levels of mRNA (D) and protein (E) of FGF7 in the histoculture 72 h after IL-17A stimulation. The protein level of FGF7 in the supernatant of the entheseal tissue was measured by ELISA. (F, G) Effect of FGF7 on the expressions of Col2A1 (F) and Acan (G) mRNA in the tissue culture. The mRNA level was determined using quantitative RT–PCR 3 h after FGF7 stimulation (10 ng/ml). (H) Effect of FGF7 on the expressions of the BMP2 protein in the culture supernatant. The protein level was determined using ELISA 24 h after FGF7 stimulation (10 ng/ml). (I) Expression of IL-17RC mRNA in the histoculture. mRNA levels were determined using quantitative RT–PCR 24 h after IL-17A (10 ng/ml) or IL- 17F (10 ng/ml) stimulation. The data presented are relative mRNA levels normalized to GAPDH. (A, B, C, D, E, I) Results are shown as the mean ± SD with individual data. *P < 0.05; **P < 0.01 by Dunnett’s parametric test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice. (D, E) *P < 0.05 by a t test or an Aspin–Welch t test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice.

    Techniques Used: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing



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    Figure <t>1.</t> <t>IL-17A</t> induced the up-regulation of endochondral ossification–related genes in the entheseal tissues. (A, B) Expressions of Col2A1 (A) and Acan (B) mRNA in the entheseal tissue culture. mRNA levels were determined using quantitative RT–PCR 72 h after IL-17A stimulation. (C) Production of the BMP2 protein in the entheseal tissue culture supernatant. The protein level was determined using <t>ELISA</t> 72 h after IL-17A stimulation. (D, E) Levels of mRNA (D) and protein (E) of FGF7 in the histoculture 72 h after IL-17A stimulation. The protein level of FGF7 in the supernatant of the entheseal tissue was measured by ELISA. (F, G) Effect of FGF7 on the expressions of Col2A1 (F) and Acan (G) mRNA in the tissue culture. The mRNA level was determined using quantitative RT–PCR 3 h after FGF7 stimulation (10 ng/ml). (H) Effect of FGF7 on the expressions of the BMP2 protein in the culture supernatant. The protein level was determined using ELISA 24 h after FGF7 stimulation (10 ng/ml). (I) Expression of IL-17RC mRNA in the histoculture. mRNA levels were determined using quantitative RT–PCR 24 h after IL-17A (10 ng/ml) or <t>IL-</t> <t>17F</t> (10 ng/ml) stimulation. The data presented are relative mRNA levels normalized to GAPDH. (A, B, C, D, E, I) Results are shown as the mean ± SD with individual data. *P < 0.05; **P < 0.01 by Dunnett’s parametric test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice. (D, E) *P < 0.05 by a t test or an Aspin–Welch t test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice.
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    Figure <t>1.</t> <t>IL-17A</t> induced the up-regulation of endochondral ossification–related genes in the entheseal tissues. (A, B) Expressions of Col2A1 (A) and Acan (B) mRNA in the entheseal tissue culture. mRNA levels were determined using quantitative RT–PCR 72 h after IL-17A stimulation. (C) Production of the BMP2 protein in the entheseal tissue culture supernatant. The protein level was determined using <t>ELISA</t> 72 h after IL-17A stimulation. (D, E) Levels of mRNA (D) and protein (E) of FGF7 in the histoculture 72 h after IL-17A stimulation. The protein level of FGF7 in the supernatant of the entheseal tissue was measured by ELISA. (F, G) Effect of FGF7 on the expressions of Col2A1 (F) and Acan (G) mRNA in the tissue culture. The mRNA level was determined using quantitative RT–PCR 3 h after FGF7 stimulation (10 ng/ml). (H) Effect of FGF7 on the expressions of the BMP2 protein in the culture supernatant. The protein level was determined using ELISA 24 h after FGF7 stimulation (10 ng/ml). (I) Expression of IL-17RC mRNA in the histoculture. mRNA levels were determined using quantitative RT–PCR 24 h after IL-17A (10 ng/ml) or <t>IL-</t> <t>17F</t> (10 ng/ml) stimulation. The data presented are relative mRNA levels normalized to GAPDH. (A, B, C, D, E, I) Results are shown as the mean ± SD with individual data. *P < 0.05; **P < 0.01 by Dunnett’s parametric test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice. (D, E) *P < 0.05 by a t test or an Aspin–Welch t test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice.
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    Figure <t>1.</t> <t>IL-17A</t> induced the up-regulation of endochondral ossification–related genes in the entheseal tissues. (A, B) Expressions of Col2A1 (A) and Acan (B) mRNA in the entheseal tissue culture. mRNA levels were determined using quantitative RT–PCR 72 h after IL-17A stimulation. (C) Production of the BMP2 protein in the entheseal tissue culture supernatant. The protein level was determined using <t>ELISA</t> 72 h after IL-17A stimulation. (D, E) Levels of mRNA (D) and protein (E) of FGF7 in the histoculture 72 h after IL-17A stimulation. The protein level of FGF7 in the supernatant of the entheseal tissue was measured by ELISA. (F, G) Effect of FGF7 on the expressions of Col2A1 (F) and Acan (G) mRNA in the tissue culture. The mRNA level was determined using quantitative RT–PCR 3 h after FGF7 stimulation (10 ng/ml). (H) Effect of FGF7 on the expressions of the BMP2 protein in the culture supernatant. The protein level was determined using ELISA 24 h after FGF7 stimulation (10 ng/ml). (I) Expression of IL-17RC mRNA in the histoculture. mRNA levels were determined using quantitative RT–PCR 24 h after IL-17A (10 ng/ml) or <t>IL-</t> <t>17F</t> (10 ng/ml) stimulation. The data presented are relative mRNA levels normalized to GAPDH. (A, B, C, D, E, I) Results are shown as the mean ± SD with individual data. *P < 0.05; **P < 0.01 by Dunnett’s parametric test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice. (D, E) *P < 0.05 by a t test or an Aspin–Welch t test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice.
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    Figure <t>1.</t> <t>IL-17A</t> induced the up-regulation of endochondral ossification–related genes in the entheseal tissues. (A, B) Expressions of Col2A1 (A) and Acan (B) mRNA in the entheseal tissue culture. mRNA levels were determined using quantitative RT–PCR 72 h after IL-17A stimulation. (C) Production of the BMP2 protein in the entheseal tissue culture supernatant. The protein level was determined using <t>ELISA</t> 72 h after IL-17A stimulation. (D, E) Levels of mRNA (D) and protein (E) of FGF7 in the histoculture 72 h after IL-17A stimulation. The protein level of FGF7 in the supernatant of the entheseal tissue was measured by ELISA. (F, G) Effect of FGF7 on the expressions of Col2A1 (F) and Acan (G) mRNA in the tissue culture. The mRNA level was determined using quantitative RT–PCR 3 h after FGF7 stimulation (10 ng/ml). (H) Effect of FGF7 on the expressions of the BMP2 protein in the culture supernatant. The protein level was determined using ELISA 24 h after FGF7 stimulation (10 ng/ml). (I) Expression of IL-17RC mRNA in the histoculture. mRNA levels were determined using quantitative RT–PCR 24 h after IL-17A (10 ng/ml) or <t>IL-</t> <t>17F</t> (10 ng/ml) stimulation. The data presented are relative mRNA levels normalized to GAPDH. (A, B, C, D, E, I) Results are shown as the mean ± SD with individual data. *P < 0.05; **P < 0.01 by Dunnett’s parametric test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice. (D, E) *P < 0.05 by a t test or an Aspin–Welch t test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice.
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    Figure 1. IL-17A induced the up-regulation of endochondral ossification–related genes in the entheseal tissues. (A, B) Expressions of Col2A1 (A) and Acan (B) mRNA in the entheseal tissue culture. mRNA levels were determined using quantitative RT–PCR 72 h after IL-17A stimulation. (C) Production of the BMP2 protein in the entheseal tissue culture supernatant. The protein level was determined using ELISA 72 h after IL-17A stimulation. (D, E) Levels of mRNA (D) and protein (E) of FGF7 in the histoculture 72 h after IL-17A stimulation. The protein level of FGF7 in the supernatant of the entheseal tissue was measured by ELISA. (F, G) Effect of FGF7 on the expressions of Col2A1 (F) and Acan (G) mRNA in the tissue culture. The mRNA level was determined using quantitative RT–PCR 3 h after FGF7 stimulation (10 ng/ml). (H) Effect of FGF7 on the expressions of the BMP2 protein in the culture supernatant. The protein level was determined using ELISA 24 h after FGF7 stimulation (10 ng/ml). (I) Expression of IL-17RC mRNA in the histoculture. mRNA levels were determined using quantitative RT–PCR 24 h after IL-17A (10 ng/ml) or IL- 17F (10 ng/ml) stimulation. The data presented are relative mRNA levels normalized to GAPDH. (A, B, C, D, E, I) Results are shown as the mean ± SD with individual data. *P < 0.05; **P < 0.01 by Dunnett’s parametric test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice. (D, E) *P < 0.05 by a t test or an Aspin–Welch t test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice.

    Journal: Life science alliance

    Article Title: FGF7 as an essential mediator for the onset of ankylosing enthesitis related to psoriatic dermatitis.

    doi: 10.26508/lsa.202403073

    Figure Lengend Snippet: Figure 1. IL-17A induced the up-regulation of endochondral ossification–related genes in the entheseal tissues. (A, B) Expressions of Col2A1 (A) and Acan (B) mRNA in the entheseal tissue culture. mRNA levels were determined using quantitative RT–PCR 72 h after IL-17A stimulation. (C) Production of the BMP2 protein in the entheseal tissue culture supernatant. The protein level was determined using ELISA 72 h after IL-17A stimulation. (D, E) Levels of mRNA (D) and protein (E) of FGF7 in the histoculture 72 h after IL-17A stimulation. The protein level of FGF7 in the supernatant of the entheseal tissue was measured by ELISA. (F, G) Effect of FGF7 on the expressions of Col2A1 (F) and Acan (G) mRNA in the tissue culture. The mRNA level was determined using quantitative RT–PCR 3 h after FGF7 stimulation (10 ng/ml). (H) Effect of FGF7 on the expressions of the BMP2 protein in the culture supernatant. The protein level was determined using ELISA 24 h after FGF7 stimulation (10 ng/ml). (I) Expression of IL-17RC mRNA in the histoculture. mRNA levels were determined using quantitative RT–PCR 24 h after IL-17A (10 ng/ml) or IL- 17F (10 ng/ml) stimulation. The data presented are relative mRNA levels normalized to GAPDH. (A, B, C, D, E, I) Results are shown as the mean ± SD with individual data. *P < 0.05; **P < 0.01 by Dunnett’s parametric test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice. (D, E) *P < 0.05 by a t test or an Aspin–Welch t test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice.

    Article Snippet: ELISA kits were used to measure IL-17A (R&D Systems), IL-17F (R&D Systems), IL-6 (R&D Systems), FGF10 (Wuhan Huamei Biotech), and FGF7 levels in the plasma.

    Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing